Overgaard, Martin Nielsen, Søren S.īackground: Tetracyclines are among the most used antibiotics in livestock worldwide. Relation between tetR and tetA expression in tetracycline resistant Escherichia coli To our knowledge, this is the first report of tet(A, tet(B, tet(M, tet(O, tet(Q, and mosaic gene tet(O/W/32/O in R. Moreover, the tet(X is the main mechanism of tetracycline resistance in R. In conclusion, the tet(A, tet(B, tet(M, tet(O, tet(O/W/32/O, tet(Q, and tet(X genes were found and conferred tetracycline resistance in R. Additionally, the tet(X gene could transfer into susceptible strain via natural transformation (transformation frequencies of ~10−6. anatipestifer ATCC 11845 transconjugants carrying tet(A, tet(B, tet(M, tet(O, tet(O/W/32/O, tet(Q, and tet(X genes exhibited tetracycline resistance with MIC values ranging from 4 to 64 μg/ml. anatipestifer isolates confirmed that tet(A, tet(B, tet(M, tet(O, tet(Q and an unusual mosaic tet gene tet(O/W/32/O were present in R. Sequence analysis of the tet gene ORFs cloned from R. One isolates, R131, unexpectedly contained three tet genes, i.e., tet(M, tet(O, and tet(X. In these tet gene positive strains, 31 (14.6%, 2 (0.9%, 5 (2.4%, 1 (0.5%, 3 (1.4% were detected containing tet(A/tet(X, tet(M/tet(O, tet(M/tet(X, tet(O/tet(X, and tet(Q/tet(X simultaneously, respectively. However, tet(C, tet(E, tet(G, tet(K, and tet(W were not detected in any isolate. Other genes, including tet(A, tet(M, tet(Q, tet(O, tet(B, and tet(O/W/32/O, were found at frequencies of 20.8, 4.7, 1.4, 0.9, 0.9, and 0.5%, respectively. anatipestifer isolates possessed the tet(X gene. The results of PCR detection showed that, 170 of 212 (80.2% R. anatipestifer isolates exhibited resistance to tetracycline (the MICs ranged from 4 to 256 μg/ml. The results showed that 192 of 212 (90.6% R. anatipestifer isolates from China between 20 was tested. Various Profiles of tet Genes Addition to tet(X in Riemerella anatipestifer Isolates From Ducks in Chinaĭirectory of Open Access Journals (Sweden)įull Text Available To investigate tetracycline resistance and resistant genotype in Riemerella anatipestifer, the tetracycline susceptibility of 212 R.
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